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I have been trying to develop a reproducible and specific method for
simultaneous surface and cytoplasmic staining. I have had problems with
most of the published methodology especially when attempting to use
an isotype control.
>>>I would also like to hear about successful methods for this-please
post to the discussion group as well! In fact, I would be grateful if
anyone can share a recipe for successful detection of intracellular
IL-4 and IFN-gamma: we have not been able to detect these (or IL-2)
using a variety of protocols on human cells in bulk culture, or in
human clones. Activation has been tried with PHA, PPD, PMA and solid
phase OKT3. Any offers?
Stan.
Stanley Ress
Clinical Immunology laboratory
H47 Old Main Bldg
Groote Schuur Hospital and UCT
Cape Town, 7925
South Africa
TEL:INTERN. + 021-7946176
FAX: " + 021-4486815
e-mail: SRESS@uctgsh1.uct.ac.za
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