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Carolyn Dawson
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REPLY FROM: Dawson, Carolyn
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Subject: 3 color phenotyping
To: cytometry@flowcyt.cyto.purdue.edu
Date: Mon, 7 Feb 1994 15:34:54 +0200 (WET)
From: Yashpal Agrawal <yagrawal@messi.uku.fi>
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Dear Dr. Langweiler,
We tried 3 color analysis on an FacStar using PerCP derivatives. No
fluorescence was observed in the Fl3 channel for the weakly positive antigens,
e.g. CD19. We contacted the manufacturer, who suggested that we change the
standard filter set to another which was more suitable. On changing the filter
set, still no fluorescence was seen. We were then told that PerCP cannot be
detected on our FACSstar, as it was not sensitive enough, since apparently
perCp can be detected on a FACScan. I dont remember clearly the situation for
the brightly staining cells. As I remember even those antigens were only dimly
fluorescent in Fl3.
It would be interesting to know whether PerCP derivatives can be measured on
a FACStar, our machine is from the year 1988/89.
-- Yash Pal Agrawal MD, PhD Dept. of Clinical Chemistry, Tel:+358-71-173155 Kuopio University Hospital, FAX:+358-71-173200 POB 1777, 70211 Kuopio Finland E-mail: yagrawal@messi.uku.fi
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