 |
 |
 |
 |
>Finally, I'd like to know what software is available (commercial or
>otherwise) to slice up the events in a single list-mode file. (I have
>some home-grown software under development if anyone is interested in
>using it.) Do any of the commercial packages (e.g. from Phoenix, Verity,
>B-D, or Coulter) do time-slicing?
Based on my experience, I would say no commercial packages handle time slicing
very well, if at all, with the possible exception of Win-List (Verity).
BD recommends using LYSYS II for kinetics analysis. LYSYS II only allows 8
regions at a time, so for a 1024 channel histogram, you would have to set up 128
region files of 8 regions each. I, for one, am not willing to spend the time
creating all those regions (you can't type them in, you have to use a mouse).
PC-LYSYS works the same way.
I've seen demos of Coulter's Elite software. I don't remember anything special
related to time-slicing. It's probably possible but awkward.
Phoenix has Multi-Time. I have a demo for an old version (1.00, I think they've
updated since then, so check for new capabilities). With v1.00, there is a
function called 'slice', which allows you to generate one a histogram at a time,
which you would then analyze and printout (didn't see an easy way to port to
spreadsheet). It would be unacceptible to me to print out 1024 pages just to
get stats and plug them right back into a spreadsheet.
Verity has Win-List. I have a demo, but haven't had much time with it. The
tutorial has an example of analyzing kinetics data and exporting to Excel. This
package is the most promising, but I have yet to put it through the paces.
I suspect that we are a small percentage of a small percentage of the flow
analysis market (i.e., not enough interest for the big companies to come up with
a product). So we're left with your MFI, my CF (runs on VAX/VMS FORTRAN, still
tidying up the background subtraction thing), and anything else 'home grown'.
When time and resources permit, I'll be writing code for MS Windows and
Macintoshes, as these are our primary analysis stations now.
Eric Van Buren e-mail: vanburen%flovax.dnet@rocdec.roc.wayne.edu
Immunology & Microbiology voice: 313 577 1009
Wayne State University fax: 313 577 1155
|
|
|
|