Kinetics, unrinsed?

ERIC MARTZ (emartz@titan.ucc.umass.edu)
07 Jan 1993 14:01:53 -0500 (EST)

Can anyone direct me to publications in which the time kinetics of
binding of directly fluorochrome-conjugated antibody to living cells
were followed by flow cytometry? The idea is to add the antibody to
the cells and then immediately begin acquisition, without rinsing.
Ideally, a single large file can be acquired over several minutes
while binding proceeds; then medians can be taken on sequential
"slices" of the data in the file. Thus, the entire kinetic curve is
generated from a single sample.

I'm particularly interested in the unrinsed mode described above, but
I'd also be interested in any publications using a more conventional
approach in which binding is stopped by rinsing at various times in
different samples, and then each sample is analyzed as a single time
point.

Finally, I'd like to know what software is available (commercial or
otherwise) to slice up the events in a single list-mode file. (I have
some home-grown software under development if anyone is interested in
using it.) Do any of the commercial packages (e.g. from Phoenix, Verity,
B-D, or Coulter) do time-slicing?

-- 
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