Re: Ethanol fixation

Micael Granstrom (mgranstrom@ume.foa.se)
Thu, 12 Sep 1996 07:16:10 +0200

Hi
I=B4m also new in this field but the method i have used for fixation is
ethanol fixation. The way I do it is as follows:
1. Centrifuge and wash cells twice with PBS
2. Pour off the PBS and resuspend the cells in the remaining drop.
3. Add 500 =B5l of Freeze-cold 95% ethanol.
4. Leave in a frezzer for 30 min.
5. Centrifuge down cells and wash twice with PBS

This is the way I do it and it works nice for me. If someone has a different
opinion about how to do it please don=B4t hesitate to correct me.

Micael Granstrom
FOA ABC-skydd
S-901 82 Umea
Sweden
e-mail mgranstrom@ume.foa.se

______________________________ Reply Separator=
_________________________________
Subject: Ethanol fixation
Author: "Bruce S. Hass" <BHASS@nctr.fda.gov> at Internet
Date: 9/10/96 10:50 PM

Hi,
I am interested in staining cells with Bodipy by the method of Gocze=20
and Freeman (Cytometry 17:151-158 1994), which calls for ethanol=20
fixed cells. Unfortunately the paper does not give details about the=20
fixation procedure. Is ethanol fixation so simple, obvious, or common?=20
Any suggestions on how to do it would be appreciated. Thanks.
=20
Bruce Hass
National Center for Toxicological Research=20
Jefferson AR 72079
(501) 543 7365
(501) 543 7745 (fax)
bhass@nctr.fda.gov=20

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