Ethanol fixation

Bruce S. Hass (BHASS@NCTR.FDA.GOV)
Tue, 10 Sep 1996 11:54:29 CST

Hi,
I am interested in staining cells with Bodipy by the method of Gocze
and Freeman (Cytometry 17:151-158 1994), which calls for ethanol
fixed cells. Unfortunately the paper does not give details about the
fixation procedure. Is ethanol fixation so simple, obvious, or common?
Any suggestions on how to do it would be appreciated. Thanks.

Bruce Hass
National Center for Toxicological Research
Jefferson AR 72079
(501) 543 7365
(501) 543 7745 (fax)
bhass@nctr.fda.gov


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