Re: Autofluorescence correction of glutaraldehyde fixed cells
Howard Shapiro (hms@shapirolab.com)
Tue, 10 Sep 1996 09:49:22 -0400 (EDT)
What Dr. Boucher may want to do is use dimethylsuberimidate instead of
glutaraldehyde as a fixative; it cross-links proteins in a similar fashion
but produces little or no autofluorescence. It has been used successfully
to do immunofluorescence flow cytometry of red cells (for example in the
work from Livermore on glycophorin mutations). My guess is that there's a
much better chance dimethylsuberimidate will work than there is of finding a
way to deal with glutaraldehyde-induced fluorescence (I'm assuming that
excitation at 488 nm is being used; red excitation might get around the
problem).
-Howard
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