RE: CyChrome & PerCP
Jose C. Segovia (segovia@CIEMAT.ES)
Tue, 10 Sep 1996 16:16:43 +0000 (GMT)
This is a small remark to the good explanations given by Calman Prussin
about CyChrome & PerCP. I only would like to point out that CyChrome (from
Pharmingen) is a little bit different to Tricolor (i.e. from Caltag) and others:
CyChrome is a tandem PE-APC
Tricolor is a tandem PE-Cy5.
There espectra are similar but, in my hands they work different depending on
the cytometer you are using. CyChrome works really fine on FACStar and
FACScan cytometers. However, is hardly to see positive events when you are
using an EPICS ELITE (675nm bp). On the contrary, Tricolor works find on the
EPICS with the same settings, and very easy to compensate. I haven't try
Tricolor on FACS ones.
Regards to all flow-ers
Jose C. Segovia
Dpt. of Molecular and Cellular Biology
CIEMAT
Av. Complutense, 22
28040 Madrid
SPAIN
Tfn : 34 1 346 6484
Fax : 34 1 346 6393
E-mail : Segovia@ciemat.es
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
