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>I have a few questions regarding filamentous algae
>FCM work is done with a Coulter Elite, equipped with a sorter.
>Let's define a filamentous alga as 2.5 X 150 micron.
>
>1. When stops a measurement? When I measure time-of-flight I get a sharp
>edge at about 40 micron. Is this also th case with with peak or
>integrated measurements or will the measurement go on until the particle
>has passed the flowcell (i.e. the signal is below the triggervalue)?
>Because of the hydrodynamical focussing a filament is supposed to pass
>longitudinally, so it should take (in this case) hundred and fifty
>microns.
Dear Hans,
It seems you have found a way to measure the low frequency cut off of your
amplifiers signal band pass: 25 KHz. Try an amplifier with adjustable
characteristics (like ortec research amp). You can increase the maximum
measurable particle length by increasing the sheath rate (signal length is
determined by particle length x velocity-1), however the fluid shear
increases simultaneously and you risc zipping the filaments. More in
Cytometry 10: 522-528,and 529-539,1989
Best wishes,
George Dubelaar
DRIE
Zeelt 2, 2411DE Bodegraven NL
dubelaar@pi.net web: http://www.pi.net/~dubelaar/drieproj.html
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