Re: Stability of PerCP -Reply
William Wansley (WWANSLEY@MAIL.MCG.EDU)
Fri, 06 Sep 1996 09:42:25 -0400
I had a similar experience. A number of years ago I tried a PerCP
labelled antibody on our 752 FC and found a tremendous amount of
photo-bleaching evan at the 50 mW level. Our BD rep. knew what
instrument we had and did not mention the bleaching problem. You
would think that the company would want to save us a lot of trouble
especially from a known problem. Oh, well, I guess it keeps us on
our toes.
>>> "BORTH NICOLE" <BORTHN@edv2.boku.ac.at> 9/5/96, 09:45am >>>
Dear Steve,
you asked for information about PerCP. We tried using it, but don't
any more, because PerCP bleaches rapidly at laser powers higher than
50mW. As we have a 5W Argon laser the lowest power we can get out of
it is 60mW. The die bleaches so quickly then, that you see hardly any
specific fluorescence. If you have a weaker laser that should be
fine, however. BD knows about this, but (at least at the time)
did not mention it in any of their product information.
Kind regards
Nicole BorthNicole Borth
University of Agriculture
Institute for Applied Microbiology
Muthgasse 18
1190 Vienna, Austria
Tel. +43/1/36006/6232
Fax. +43/1/369 7615
email: borth@mail.boku.ac.at
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