Re: Rhodamine123 & PE

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> Has anyone sorted hematopoetic stem cells using rhodamine123 and another
> color like PE, FITC etc off an Argon laser? What kind of compensation
> problems should one expect? Any references out there?

Yes. You will see Rh123 in your normal FITC channel (? "FL1"). It can
be used in combination with PE (? "FL2") with a moderate (but workable)
increase in the FL2-%FL1 compensation. You may find it brighter than
FITC immuno-fluorescence.

For refs, try Li and Johnson: Blood 85(6) 1472-9 '95,
Blood 84(2) 408-14 '94
J. Exp Med. 175(6) 1443-7 '92

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