endothelial cell adhesion molecules
kristen Aherne (kma129@psu.edu)
Tue, 23 Jul 1996 14:42:34 GMT
I am looking for protocols to analyze VCAM-1, ICAM-1, and ELAM-1 on bovine
endothelial cells by flow cytometry. I am particularly interested in any
known cross reactivity between human and bovine antibodies. In addition, I
am interested in methods of harvesting the cells for transfer to the flow
cytometry facility, as I fear that trypsinization will damage the adhesion
proteins. Please send any suggestions or methodologies. Thank you.
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
