re: endothelial cell adhesion molecules
Paul Sopp (paul.sopp@bbsrc.ac.uk)
Wed, 24 Jul 96 10:00:49 +0100
>I am looking for protocols to analyze VCAM-1, ICAM-1, and ELAM-1 on bovine
>endothelial cells by flow cytometry. I am particularly interested in any
>known cross reactivity between human and bovine antibodies. In addition, I
>am interested in methods of harvesting the cells for transfer to the flow
>cytometry facility, as I fear that trypsinization will damage the adhesion
>proteins. Please send any suggestions or methodologies. Thank you.
At the time of the 3rd Bovine Leukocyte Antigen Workshop (Davis, Ca 1995)
there were no characterized mAbs to bovine VCAM-1, ICAM-1 or ELAM-1. Many of
the mAbs submitted to the 5th and 6th Human Workshops were also tested and
most were negative on bovine cells. We have some data suggesting that one or
two human VCAM-1 mAbs may cross-react. Trypsin will damage some proteins so
we routinely use cell dissociation fluid (non-enzymatic, Sigma) but cannot
guarantee that this will not affect the molecules you are interested in.
Paul Sopp,
Institute for Animal Health,
Compton,
UK.
sopp@bbrsc.ac.uk
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