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>
> Hello Everyone!!
>
> Our institution recently purchased a FACS calibur. It has been in use for
> the past three weeks and we have had very few days where there has not been
> either an instrumentation problem or a (Cellquest) software problem.
>
> Personally, I do not have very much experience with BD flow systems as I am
> more of a "Coulter" person. I am wondering if others have had similar
> problems with new instruments. Did we get a "lemon" or is this par for the
> course?
>
> One difference between this instrument and others that are used here is that
> is a multi user instrument. There is no dedicated operator and that could
> very well be part of the problem.
>
> Anyone out there had similar experiences??
>
> Abby Allen
> Center for Blood Research
> Boston, Ma. 02115
>
> aallen1216@aol.com
>
Dear Abby:
I need to emphasize that what you are experiencing
is not "par for the course" with respect to BD. They are
much much better than that! I can almost assure you that
the crux of the problem is that it is a multi-user instrument.
I am aware that the MacIntosh platform does have it's own
set of quirks but not to the extent to which you are describing.
I also realize that the Coulter and BD engineering philosphy
differ and that may take some time getting comfortable with
but the "Lemon" theory cannot be discounted. Get BD involved!
They should give you insight as to the source, user or instrument,
of your difficulties.
Gilbert
Gilbert Radcliff
Coordinator, Flow Cytometry Core Facility
H. Lee Moffitt Cancer Center & Research Institute
12902 Magnolia Drive
Tampa, FL 33612
Phone: 813-972-8400 ext. 2005
Fax: 813-979-6700
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