re:long time storage of fixed cells
ERNEST STAPLETON (kstaplet@morgan.ucs.mun.ca)
Fri, 14 Jun 1996 20:10:31 -0400
Hello, from the most easterly flow lab in North America.
Our experiences with long term storage of fixed cells
(1% paraformaldehyde in pbs) has been good. When our 100mw argon laser
gave up, Becton Dickenson couldn't replace it for 2 weeks. Between the
jigs and the reels 3 weeks went by before we started running the
backlog of samples. All the oldest samples had slightly grungy scatter
patterns and the fluorescence (fitc and pe) histograms had lower
background fluorescence than we thought they would have. There were
no problems with the data analysis. All types of specimens were
analysed, bloods and marrows lysed and fixed with facslyse (BD), blood
lysed with "cytolyse" from Cytek,lymph nodes and other tissues fixed
with 1% paraformaldehyde in pbs. There were all types of clinical
samples: CLL, CML, AML, lymphomas etc. and we didn't see any cell loss
or degradation of any single type of cell in our preparations. We were
happy.
I hope this information will help you and best of luck, Ruedi. It
sounds like an interesting project!
Ernest Stapleton
Chief Technologist, Clinical Immunology Lab
General Hospital Health Sciences Centre
St. John's, Nf. Canada
A1B 3V6
phone:709-737-6677
fax:709-737-6395
e-mail:kstaplet@morgan.ucs.mun.ca
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If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
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EMAIL robinson@flowcyt.cyto.purdue.edu
