RE: fixing without leakage

Calman Prussin (CPRUSSIN@atlas.niaid.nih.gov)
Mon, 17 Jun 1996 08:43:25 -0400

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My impression is that paraformaldehyde fixation does not permeabilize or
at least does not permeaboilize efficiently. I guess the real test would
be to try to stain for an intracellular protein, say actin, without
permeabilization.

Calman Prussin

>----------
>From: Deborah Berglund
>Sent: Friday, June 14, 1996 2:26PM
>Cc: Cytometry Mailing List
>Subject: Re: fixing without leakage
>
>
>Hi everyone,
>
>We want to be able to fix (stop metabolic action, etc.) some cells in a
>
>monolayer. All of the fixatives I am familiar with permeabilize the
>cells and we can't have that! Any ideas?
>
>Going into the mountains tomorrow with llamas carrying all my gear!
>Luxury or lazy?
>
>Deb Berglund
>Montana State University
>Bozeman, MT 59715
>
>Montana, no longer in the nightly news due to FREEMEN
>
>

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu