Calculation of C classes in endoreduplicating nuclei

Gerard J Engelen-Eigles (enge0019@gold.tc.umn.edu)
Tue, 10 Oct 1995 21:34:31 -0500 (CDT)

Dear colleagues,

I'm working on DNA endoreduplication in maize nuclei, analyzing the
tissue with a Coulter Epics flow cytometer/MDADSII. I want to calculate
the percent nuclei in each individual C class to see how
endoreduplication progresses as the tissue get older--e.g. how many
nuclei are in the higher C classes over time. Are there any statistical
programs to determine the number of cells/percentage of cells in each
individual C class/peak?

Thanks in advance,

Gerard Engelen-Eigles
Dept. Agronomy, university of MN, St. Paul, MN
enge0019@gold.tc.umn.edu


Home Page Table of Contents Sponsors Web Sites
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu