RE: Fish antibodies? and Thanks....

Ross Longley (Longley@HBOI.EDU)
Wed, 11 Oct 95 08:47:00 PDT

Steve: No, not me! We have been looking at fish leukocytes only from the
standpoint of DNA content with P.I. I might pass along, however, that we
routinely use DAPI to stain oyster larvae DNA. We get very good histograms
with it, no change in staining characteristics with time (several hours for
each run of around 70-80 samples. The oyster sperm we use for a standard,
remains rock solid in as far as the fluorescence does not diminish or
increase with time. One thing we always do, however, is to allow the sample
to run about 1.0 minute before data collection--just to ensure that
everything stabilizes and their is no inadvertant "leaching" of the dye into
the plastic tubing. It also helps to tap the machine with a "magic wand"
before you begin!

--Ross--

____________________________________________________________________________
_______
Ross E. Longley, Ph.D.
Immunology and Tumor Biology Group Leader
Harbor Branch Oceanographic Institution
5600 U.S. #1,North
Fort Pierce, FL 34946
Phone: (407) 465-2400, Ext 486
FAX: (407) 465-1523
eMail: longley@hboi.edu
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From: owner-cyto-sendout
To: Cytometry Mailing List
Subject: Fish antibodies? and Thanks....
Date: Tuesday, October 10, 1995 4:04PM

Thanks to all who responded re: my weird DAPI observation--sometimes
I can be amazingly dense (smacking forehead w/ palm).

I would normally check the archive, but my browser's broken-- someone
in this group mentioned antibodies against fish (piscine)
leukocytes--Ross Longley maybe? Could you drop me a line if you know
anything about the subject?

Thanks, (as always)

Steve
*********************************************************
Steve G. Hilliard Life's too short
Cell Analysis Facility to drink bad beer!
University of Georgia

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