Aneuploidy and Polyploidy
David Gebhard (gebhard@aecom.yu.edu)
Fri, 8 Sep 1995 16:53:39 -0400
Over the past 18 months, there has been a remarkable
increase in the amount of DNA cell cycle analysis being requested of the
FACS Core. None of the samples have been clinically related, all in
support of basic reseach programs, ie., tissue culture, primary culture,
mostly murine, rat etc....
Question....I regularly report back that there
appears to be a aneuploid population growing with the primary population,
usually a tetraploid component. I once had several lines of Raji cells to
compare, one had become completely "tetraploid" from the others. Is this as
widespread observation? How much of a concern is it to investigators
looking for shifts in cell cycle proportions,
apoptosis, etc. With transfected lines, would there be a "doseage" effect
in the aneuploid cells making them more or less responsive to the effect in
question?
Would it be useful to compare eg., a murine line with
normal mouse cells for an initial measure of aneuploidy?
I now have an investigator screening lines for
polyploidy to find a "good" one to continue his work.
Am I seeing things?..... Thanks..... Dave G.
Dave Gebhard
Director FACS/ Oligonucelotide Synthesis Facilities
Albert Einstein College of Medicine
1300 Morris Park Avenue
Bronx, New York 10461
718-430-2724/ 3573
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
