Re: Keeping large cells in suspension

Alan Bishop (BISHOP@immuno.imvs.sa.gov.au)
Fri, 11 Aug 1995 11:08:30 GMT+1030

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Maybe in reply to: gap@MIT.EDU: "Keeping large cells in suspension"

Its been a while, but 15 years ago (when I was at the Sir william Dunn
School of Pathology in Oxford) we used to sort rat fetal liver and
bone marrow cells suspended in Percoll to stop them settling and
claging the system. There were no observed deterimental effects to
the cells (or the Facs II) and we reduced our blockage rate from more
than 3 or 4 times a sort to ZERO.

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Next message: Eric Miller: "Re: HP Consort 32 Data Storage / Histogram Deconvolution"
Previous message: Anna Doubrovsky: "FACSCalibur"
Maybe in reply to: gap@MIT.EDU: "Keeping large cells in suspension"

CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu