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PI & FITC ON THE ELITE

You do not say which configuation you are using for your optical
filters. If you are using PI to measure the cell cycle, it is best to
set the discriminator on red fluorescence. This would usually be on
PMT4 but depends on the filter layout.

If you are using PI to discriminate between dead and live cells,
you should trigger on forward scatter.

Best wishes,

Mike Ormerod

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu