Cells-on-beads
Brian Smith (smith@beaker.med.yale.edu)
Tue, 6 Jun 95 11:20:08 -0400
We tried measuring HLA-Dr on macrophages adherent to beads many years ago
with reasonably OK success - although not enough success to make us want to
do this forever (now we use an ACAS 570 microscope based laser cytometer
that permits measurements and sorting of adherent cells). The reference is
Cytometry 3: 449-52, 1983.
-Brian
>We are planning to try to analyze integrins on the surface of cells grown
>on beads. We would like to be able to label the integrins that are being
>used by the cells to adhere to the beads while they are still adhering to
>the beads.
>Anyone ever tried anything like this? Like with superpermeable beads?
>
>Also, what is the upper size limit for analysis with FACScan? All we care
>about are the fluorescence signals.
>
>Thanks in advance.
>
>Debbie Berglund
>Microbiology
>Montana State University
>Bozeman, MT 59717
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