None
Edward Srour (edward_srour@iucc.iupui.edu)
Thu, 25 May 1995 16:20:07 -0500
Steve Gore responded on May 25, 1995 to a Wed May 24, 1995 inquiry about Go
cells from bpijb@picr.cr.ac.uk indicating that rhodamine 123 can be used to
isolate viable marrow or cord blood cells Go cells. Although I agree with
Steve that rhodamine 123 can be used to isolate "quiescent" hematopoietic
cells (and we have published extensively on that; Cytometry 12:179-83, 1991;
Blood 81:661-9, 1993; Exp
Hem 22:215-22, 1994), these cells are not necessarily in Go. Since R123
stains the mitochondrial membrane, the only thing one can say about these
cells is that they are quiescent and most likely in the Go/G1 phase of cell
cycle. However, whether these cells are in Go or G1 cannot be determined with
rhodamine 123 only. If Hoechst 33342 is added to R123 staining then the
status of these cells could be determined more precisely. Wolf et al used
that in the murine system (Exp Hem 21:614-22, 1993) and we have used it on
human cells (Exp. Hem 22:754, 1994). We will publish shortly on a new
approach to sort viable hematopoietic cells in Go using both DNA and RNA
stains to select for cells that are indeed in Go at the time of isolation.
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
