Apoptosis among CD34+ cells

STEVEN DAVID GORE (sdgore@welchlink.welch.jhu.edu)
Fri, 26 May 1995 09:20:13 -0400 (EDT)

We have been trying to use the TUNEL assay to identify apoptosis in
normal human CD34+ cells double labeled with PE-labeled anti-CD34
antibody (antibody staining before formaldehyde fixation; we have tried
both EtOH and MeOH for the secondary fixation and permeabilization step).
While this is effective for CD34-expressing cell lines, such as TF-1 and
KG1a, in isolated human bone marrow CD34+ cells, the CD34 staining seems
to disappear after the alcohol fixation step- actually down to background
levels.

Anyone have any experience or suggestions?

Steve Gore
Johns Hopkins Oncology Center
sdgore@welchlink.welch.jhu.edu

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