Apoptosis in CD34+ cells

STEVEN DAVID GORE (sdgore@welchlink.welch.jhu.edu)
Thu, 25 May 1995 20:43:34 -0400 (EDT)

We have been trying to adapt the TUNEL assay to identify apoptosis among
normal human CD34+ cells, double labeled with PE-labeled CD34 antibody.
While this procedure works well for CD34-expressing cell lines, such as
TF-1 or KG1a (the CD34 labeling performed before formaldehyde fixation,
then followed by MeOH or EtOH etc), among isolated bone marrow CD34+
cells, CD34 appears undetectable after the alcohol second fixation step
(either alcohol). Does anyone have any experience or suggestions?

Steve Gore
Johns Hopkins Oncology Center
Oncology 2-109
600 N. Wolfe St.
Balto, MD 21287-8963
sdgore@welchlink.welch.jhu.edu
410-955-8781
FAX 410-614-1005

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