Quantifying DNA
Lisa M Miller (lmmiller@morgan.ucs.mun.ca)
Fri, 10 May 1996 12:05:30 -0230 (NDT)
I am presently working on a project examining total DNA content
in apoptotic cells. In the past, I have used propidium iodide
and flow cytometry to quantify DNA. Now I am trying to find another
method to detect even smaller quantities of DNA. I recently read a
paper that used the dyes TOTO-1 and YOYO-1 in fluorometric assays to
determine DNA content. I also read that these dyes can be used with a
confocal scanner, but unfortunately we do not have a confocal scanner
here. I once read a paper mentioning the use of these dyes in flow
cytometry so I was wondering if anyone could tell me if these dyes can
really be used with a flow cytometer and if so, where I may be able to
find a protocol for their use. Molecular Probes, Inc. have sent me a
technical sheet but it too gives a protocol for their use with a
confocal scanner. I would greatly appreciate any information anyone
could tell me about this. Thank-you!
Lisa Miller Memorial University of Newfoundland Health Sciences
Centre St. John's, Newfoundland
lmmiller@plato.ucs.mun.ca
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
