Re: Sorting Sheath Fluid
dick stovel (STOVEL@Darwin.Stanford.EDU)
Fri, 12 Apr 1996 17:01:03 -0700 (PDT)
Lorie Miller asks:
> We normally sort using Haema-Line 2 as our sheath fluid in our
> FACStarPLUS. One of my users needs to sort but cannot have potassium
> or phosphate in the system. Any suggestions?
>
> What concentrations of NaCl or anything else are necessary for proper
> charging of the drops for sorting?
We used normal saline (.9% - same stuff they plugged all the hospital
patients into) happily for many years before switching to PBS
(Phosphate Buffered Saline) for our sheath fluid.
As for concentration of NaCl for electrical conductivity for sorting - not
to worry. Water with 1/50th of the concentration of normal saline will
happily conduct your drop charges.
Richard Stovel stovel@darwin.stanford.edu
Herzenberg Laboratory and Stanford Shared FACS Facility
Stanford University, Stanford CA
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