 |
 |
 |
 |
We are interested in knowing if someone has experience using TO-PRO-3 on
chord blood, bone Marrow or peripheral blood studies.
If so, have you used it with other fluorochromes?
Are their any advantages/disadvantages or problems in its use?
Thank you in advance,
Mike and Barb
Stem Cell Research Lab
American Red Cross
Portland Oregon
We are using TO-PRO-3 routinely for DNA content measurements in =
combination with two monoclonal antibodies (indirectly labeled with FITC =
and PE). When we started this type of triple labeling experiments we =
tried to combine FITC, PE and propidium iodide as well. However, using =
20 - 40 microgram/ml of PI, we had to compensate so much that we didn't =
trust the DNA histogram calculations anymore. Now we are using TP3 =
instead. The only disadvantage of the dye is the need for a second =
laser, a He-Ne laser for excitation at 633 nm. We use the standard =
coulter 675 nm BP filter for collection of the TP3 emission.
The technique has been described in a publication in Cytometry, 1994, =
17: 185 - 189.
We have applied the technique mainly to skin research. Examples can be =
found in: Acta Derm Venereol (Stockh) 1995; 75, 381 - 385 and 437 - 441 =
and very recently in Arch Dermatol Res 1996, 288, 203 - 210.
=20
----------------------------------------------
Piet van Erp
University Hospital Nijmegen
Department of Dermatology
P.O. Box 9101
6500 HB NIJMEGEN /The Netherlands
Voice: +31 (24) 3616409
Fax: +31 (24) 3541184
e-mail: p.vanerp@derma.azn.nl
|
|
|
|