Re: IgM antibodies and ugly histos

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Unfortunately,=20
>all the positives are simply wider smears out to the 2nd or 3rd=20
>decade, and still overlapping the negative significantly.

Maybe that's the way they are or..

You could try centrifugating the IgM hard (eg 100 000g for 10 minutes)=20
to get rid of aggregates of antibody. In my former lab I used an airfuge=20
that reportedly would get those g numbers. Nowadays I do 40-50 000g for=20
a bit longer to lower background on home labelled antibodies.

Mikael

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