IgM antibodies and ugly histos
Donald E. Mosier (dmosier@scripps.edu)
Wed, 28 Feb 1996 14:49:52 -0800
Some IgM antibodies have the nasty property of cryoprecipitation...when
stored at 4=B0 C. for short periods of time (like overnight), they form larg=
e
multimers that might give the observed messy histograms. A short
high-speed spin will remove aggregates, but you will then need to use much
higher concentrations of the deaggregated antibody, and the same problem
will recur the next time you store the antibody.
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
