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>How does the SEM affect the dead time?
I don't know that the actual instrument "dead" time changes however the drop
delay offset must be increased on the card, and we had to do a fair bit of
testing to get the optimum drop delay; in our hands 1.5 less than calculated.
The biggest pain on our system using the SEM is to do single cell deposition
work, where the operator must go into LYSYS II, exit to "alternative
software" then run the ACDU software, reset the drop delay, and the drop
delay offset. If the ACDU software crashes (could that actually happen,
perish the thought!) then you have to go and fire the system up again, back
through LYSYS and so on..
As to your question regarding purity etc it seems to give at least as good
results as we got pre SEM and generally better, but then we were interested
in polygonal gates...
Hope this helps,
Geoff
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Geoffrey Osborne | ____ __ o Ahh!
Flow Cytometry (FACS LAB) | __ `\ <,_
John Curtin School of Medical Research, | __ (*)/ (*)
Australian National University, | ==============|
CANBERRA, AUSTRALIA. | |--|
Email: Geoff.Osborne@anu.edu.au | |--|...
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