simulateous antigens plus DNA
kweber@vs4.im.med.umich.edu
Thu, 27 Apr 1995 16:53:37 -0400
To add to the discussion of Dr. Shankey's...
We have also been doing FITC, PE and PI on and Elite and we have found that
the further you go into the red away from the PE the better off you are in
terms of compensation. We have tried a 675 bp filter for the PI and it
improves the subtraction problem (not eliminate) and recently we purchased
a 685+/-5 nm filter to try also. Additionally, a very narrow bandpass +/-5 nm
for the PE helps a lot.
Kris Weber
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
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If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
