Re: Green Fluorescent Protein
Alice L. Givan (Alice.L.Givan@Dartmouth.EDU)
27 Apr 95 17:44:14 EDT
---Kier Watkins wrote:
Someone has asked me about using Green Fluorescent Protein for flow analysis
of transfected sperm.
What's the excitation/emission?
--- end of quoted material ---
I'm not sure whether the puns are intentional or not.... but I'll pretend I
didn't notice them. There is a paper in Science 263:802(1994) by Chalfie et al
that shows GFP spectra. Excitation has a peak at 395nm with a shoulder at
470nm and fluorescence has a peak at 509nm with a shoulder at 540nm.
Alice Givan
Englert Cell Analysis Laboaratory
Dartmouth Medical School
Lebanon, NH 03756
USA
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