Re: Green Fluorescent Protein
Doug Terle (douglas@helix.nih.gov)
Fri, 28 Apr 95 08:59:11 -0400
>A while back there was some discussion about "Green Fluorescent Protein".
> Someone has asked me about using it for flow analysis of transfected sperm.
>
>What's the excitation/emission?
>
>Kirk Watkins
>BDIS Sales
>St. Louis
Purified green fluorescent protein (GFP) absorbs blue light maximally at 395
with a minor peak at 470 and emits at 509 with a shoulder at 540.
For more information see:
1). Green Fluorescent Protein as a marker for gene expression. Chalfie, et al.
Science ( 263) pp 802-5
2) Wavelength mutations and posttranslational autoxidation of green
fluorescent protein. PNAS 91 (26) 12501-4
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
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If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
