Re: Acrosome staining

Stefan Burde (sburde@pathology.rochester.edu)
Wed, 12 Apr 95 16:41:30 -0500

>Date: Tue, 11 Apr 95 11:57:42 MDT
>From: "Michael Fox" <MFox@vines.colostate.edu>
>To: cytometry@flowcyt.cyto.purdue.edu
>Subject: Acrosome staining
>
>I have a user who is looking for a dye or dyes that are specific for the
>sperm acrosome. Has anyone out there been doing work in this area, and have
>some suggestions for possible dyes? Thanks for any input.
>
>Mike Fox
>Colorado State University
>Phone: 970-491-7618
>FAX: 970-491-0623

Mike,
Pisum sativum agglutinin (PSA) reacts with acrosome contents of acrosome-intact
sperm (Cross et. al. Gamete Res. 15:213-26, 1986). It's available conjugated to
FITC (Sigma L0770). See also a paper we have published using it for
simultaneous assessment of sperm viability and acrosome status with
single-wavelength fluorescence microscopy (Centola et al. Molecular Reproduction
and Development 27:130-5, 1990).
Hope this is helpful,
Stefan

Stefan Burde .......
University of Rochester Medical Center ******.....
Department of Pathology **..****.....
601 Elmwood Avenue, Box 626 ***..***.......
Rochester, New York 14642 *******...**...
Phone: 716.275.8804 *****....**..
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e-mail: sburde@pathology.rochester.edu *******

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