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> First, let me thank everyone who has been helping us out with our questions
> on CellQuest versus Lysys - one message here is worth dozens of phone calls!
>
> Second, we are exploring our options for re-configuring our clinical
> operation (as opposed to our research lab) and have begun looking at the
> Coulter XL with the autoloader. We have liked what we have seen so far but
> some sites have talked about having difficulty setting fluorescence
> compensation properly so that it works on all samples - this is said to be a
> possible problem for simple FITC/PE as well as more complex arrangements. I
> have not tried this myself yet but I am wondering if anyone else has seen a
> problem and, if so, if you know its etiology. We did note that some samples
> we gave to another site to look at with an XL had what appeared to be some
> problems in this realm although I initially attributed it to a few "weak"
> MoAbs coupled with setting comps based only on beads. Any comments (or any
> general comments on the Coulter) would be greatly appreciated.
>
>
Hello Brian:
I have used the Coulter XL-MCL (autosampler) for the last year and
love it. I used the FacScan for 6 months. There is no comparison. The
Coulter XL-MCL wins hands down. I have no problems with color
compensation in a standard protocol, however if you change any PMT
setting it will mess up the compensation. If you change the stock of your
dye it may effect your compensation. If the protocols are the same and
sample types are similar there are no problems. In general if you change
species, cell type, dye lots or PMT energy it is wise to run a test to
optimize your color compensation. I hope this helps. > >
If you have any questions about the Coulter XL-MCL and the
reseach support team that makes up Coulter, I would be glad to share my
experiences with anyone interested.
Lisa M.J. Smith, Ph.D.
Biomedical Sciences, OVC
Univ. of Guelph
Guelph, Ont. N1G 2W1
lsmith@uoguelph
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