addendum to nuclear antigen staining question
Raymond B. Hester (rhester@jaguar1.usouthal.edu)
Mon, 13 Feb 1995 17:49:03 -0600 (CST)
In addition to the question of methanol-only versus methanol plus Triton
X-100, does the length of wash after staining matter? One paper I ran
across indicated they washed extensively (three 20 min rinses in PBS)
when staining nuclear antigens, compared to our 1 min wash.
If we washed more extensively/longer, would it help lower non-specific,
cytoplasmic
binding (although we do have 1% BSA in all of our incubation steps) and
maybe make the nuclear staining more prominent?
Thanks again.
ray
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