file compatability

Evenson, Don Chem (evensond@mg.sdstate.edu)
Mon, 13 Feb 95 11:27:00 cst

Dear Wendy:
Your ACQCYTE acquired list mode data should be readable by LISTVIEW
software from Phoenix Flow. If you experience difficulties, please feel to
write or call. Good Luck.
Don Evenson
Dept. Chemistry, ASC 136
South Dakota State University
Brookings, SD 57007
605-688-5474 FAX 605-688-6295
email: evensond@mg.sdstate.edu
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From: Wendy Jones <wjones@uhunix.uhcc.Hawaii.Edu>
To: Steve Kelley <kelley@flowcyt.cyto.purdue.edu>
Subject: file compatability
Message-Id: <Pine.SUN.3.91.950209155437.23540B-100000@uhunix3.uhcc.Hawaii.Edu>
Mime-Version: 1.0
Content-Type: TEXT/PLAIN; charset=US-ASCII
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I'm having a file compatability problem that hopefully, someone out there
in the net can help me with.

A short time ago I had the opportunity to train in a flow lab on the mainland
(I'm in Hawaii). During my training I ran plant nuclei stained with PI
for DNA content analysis. The instrument was an EPICS, and histograms
were generated using ACQCYTE software (Pheonix) on a PC. The files were
saved in lis mode, not as histograms. NOw that I'm back home I'd like to
do something with those files. Hence my problem. The available software
here is mcycle or cytocell, neither of which appear to like my files.

Any suggestions? (besides start over)
The files are in FCS 1.0 format (ortho not MADS, if thats of help)
the parameters were LFL,FL and FS.

Thanks,
Wendy


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