Protease digestion

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Steve-
I have been having a difficult time with background when I use my .221 LCL
cells (lymphoblastoid cell line irradiated to not express MHC antigens). I
found a paper by Peter Lobo et al. (Transplantation 23:16, 1977) in which
they use protease to decrease background when using B cells for flow
cytometry. The pronase he used is no longer available, and he is out of the
country for a while. I tried using a similar protease, but it destroyed my
cells. Do you have any experience with this or do you know anyone that does?

My address is lynn_westphal@quickmail.ucsf.edu
I really appreciate any advice you have.

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu