CRBC as an internal DNA standard
Wendy Jones (wjones@uhunix.uhcc.Hawaii.Edu)
Mon, 25 Jul 1994 15:26:27 -1000
-Hopefully someone out there can give me a few suggestions about the use
of chicken red blood cells as internal DNA standards for plant nuclear
DNA samples. I'm just starting up grad reserch project looking at orchid
DNA and I've run into a slight road block. Most of the lit regarding the
methods I want to use call for fresh chicken blood. Since our university
has recently phased out all poultry research, I don't have a local source
for CRBCs. So far, the only commercially available CRBCs I've run across
are gluteraldehyde fixed. Besides being expensive, mostly due to air
freight, I'm worried that the results won't be comparable to those of other
studies. If anyone out there has any comments about using glut-fixed
CRBCs, or know of a commercial source for unfixed CRBCs I would
appreciate hearing from you.
Sincerely,
W. Jones
University of Hawaii
Dept. of Horticulture
wjones@uhunix.uhcc.hawaii.edu
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