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> Greetings:
>
> I am preparing to begin a project to measure LDL endocytosis by flow
> cytometry. I'm considering buying BODIPY labelled acetyl-LDL from
> Molecular Probes. Has anyone had good luck with this reagent? Does
anyone
> know of a better source? I plan to use 488 nm for excitation. Is
BODIPY
> better than DiI at that wavelength? Thanks in advance for any advice
you can
> offer.
>
> ---
>
>>*<<>>*<<>>*<<>>*<<>>*<<>>*<<>>*<<>>*<<>>*<<>>*<<>>*<<>>*<<>
>*<<
> Jeff Clapper, Assistant Scientist (Office) 515-294-2472
> Cell and Hybridoma Facility, 1104 MBB (Lab) 515-294-8504
> Iowa State University, Ames, IA, 50011 (FAX) 515-294-0453
>
>
>
>
The most simple and also cheap source is to prepare DiI-LDL in
your own lab. A paper describing the use of DiI-LDL in comparison
to 125I-LDL and an anti-LDL-receptor mAb was recently published
by our lab (G. Schmitz et al.: Arteriosclerosis and Thrombosis
13: 1053-1065, 1993). I am not aware of any specific advantage
of the use of Bodipy compared to DiI.
Gregor Rothe
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Institute for Clinical Chemistry and Laboratory Medicine
University of Regensburg
D-93042 Regensburg, F.R. Germany
Tel. +49 (941) 944-6208, or -6233
Fax +49 (941) 944-6202
Internet: Gregor.Rothe@KLINIK.UNI-REGENSBURG.DE
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