LDL Endocytosis

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I am preparing to begin a project to measure LDL endocytosis by flow
cytometry. I'm considering buying BODIPY labelled acetyl-LDL from
Molecular Probes. Has anyone had good luck with this reagent? Does anyone
know of a better source? I plan to use 488 nm for excitation. Is BODIPY
better than DiI at that wavelength? Thanks in advance for any advice you can
offer.

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Jeff Clapper, Assistant Scientist           (Office) 515-294-2472
Cell and Hybridoma Facility, 1104 MBB          (Lab) 515-294-8504
Iowa State University, Ames, IA, 50011         (FAX) 515-294-0453

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu