Re: flow cytometry
Kevin Becker - Phoenix Flow Systems (phnxflow@crash.cts.com)
Tue, 15 Mar 1994 11:40:02 -800 (PST)
Rita, I just wanted to reply to you about CD33 in normal blood. Off
hand, I can't remember reading about any actual studies that were
done to establish normal ranges. However, you may want to take a look at
the book from the 4th HLDA Workshop. They usually have some sort of expected
ranges for the different CD's (I don't have access to tell you for
sure). Also, I know that Cytometry Associates uses CD33 in their
leukemia panels. You may wish to contact them for some help.
About your Q-Prep question, in my experience with it, I cannot say that I
ever saw a difference in fluorescent intensities as compared to my
non-Q-preped samples (on facsan, Cytofluorograf or profile instruments)
however, I did fix my non-Q-preped samples with paraformaldehyde which
could make an intensity difference if you are not doing the same since
immunoprep does have 2%paraform. in it. I'm sure that you have already
asked your Coulter tech-rep, but if not, see what they have to say.
Sorry I don't know anything about buffalos or white rhinos, but I hope
this was a little help.
Margie Becker
Phoenix Flow Systems, San Diego, CA
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
