flow cytometry
rita@mcd4330.medunsa.ac.za
Mon, 14 Mar 94 13:45:21 +0000
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I have a number of queries and I hope someone can help:
(I have an Elite FCM)
1.Does anyone know of someone who has done lymphocyte transformation
tests (or are doing it) on animals, especially buffalo and white rhinos -
not necessarily on FCM.
2. Does anyone have experience with CD33 in normal bloods - neutrophils,
monocytes included. What is the normal range? Can anyone give me a
possible explanation why it seems as if cells prepared by Q-Prep seems to
fluoresce brighter than cells stained with antibodies without Q-Prep?
3. I have started with the analysis of Calcium content in peripheral
blood cells - anyone with tips or suggestions or advice about pitfalls?
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
