CD34+ cells in blood
Tom Mchugh (mchugh@jekyll.ucsf.EDU)
Thu, 10 Mar 1994 17:12:48 -0800
We are trying to determine the lower limit of detection of CD34+ cells in
peripheral blood. In peripheral blood samples from healthy controls we detect
from 0 - 0.5% CD34+ cells. These cells appear to be mononuclear and have
FSC/SSC patterns of lymphocytes. In the patients in which we want to harvest
peripheral stem cells the % CD34+ cells is used to calculate the total needed
for transplantation. Most reports I have seen show a low (1 - 5%) level of
CD34+ cells in bone marrow from healthy controls and the few reports I have
seen do not indicate what the "reference range" of these cells is in
peripheral blood.
We are staining with BD's anti-CD34 PE along with a bunch of other antibodies,
lysing using ammonium chloride, gating on all cells, using a FACScan and
displaying data as PE fluorescence versus side scatter.
Does anyone know how many CD34+ cells one would expect to see in a "normal
population" in the peripheral blood?
(we have tried a number of different anti-CD34's and about 4 different lysis
techniques)
mchugh@jekyll.ucsf.edu
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