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Yashpal Agrawal (yagrawal@messi.uku.fi)
Wed, 16 Mar 1994 15:20:21 +0200 (WET)
Hello!
I am relatively new to sorting. We use a FACSStar machine. When I test
recovery using standard beads, I get good recovery based on a slide test.
However,under the same conditions when I try to collect myeloma cells in
G1 phase (PI staining) by DNA staining the recovery is very poor, even though
I collect upto a million cells for DNA extraction.
I use glass tubes for collecting the sorted cells, i.e. I think the glass tubes
do not pick up a charge as do plastic tubes.
What might be the problem(s)?
--
Yash Pal Agrawal MD, PhD
Dept. of Clinical Chemistry, Tel:+358-71-173155
Kuopio University Hospital, FAX:+358-71-173200
POB 1777, 70211 Kuopio Finland E-mail: yagrawal@messi.uku.fi
--
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