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> Has anyone had any luck cloning directly on a slide for FISH analysis?
> If so, what are you using for sheath fluid? We routinely use an isotonic
> sheath fluid and expect this would leave too great a salt deposit. Any
> suggestions welcomed.
>
> Thanks,
>
> Geri Pingul
> Flow Cytometry
> Fred Hutchinson Cancer Center
>
>
>Let me clarify what we would like to do. Sort cells onto a slide to be
used as a target for FISH analysis. We have a very high cell loss
when sorting into tubes and following with a cytoprep and are looking for
an improved procedure.
Thanks again,
Geri
>
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