Fluorescence

• Stokes Shift

  • is the energy difference between the lowest energy peak of absorbance and the highest energy of emission

495 nm

520 nm

Stokes Shift is 25 nm

Fluorescein

molecule

Fluorescnece Intensity

Wavelength

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Fluorescence

This slide covers Stokes shift. This slide relates how the illumination source excites the fluorescent molecule and then how the fluorescent molecule emits the absorbed energy.