PPT Slide

Properties of Fluorescent Molecules

• Large extinction coefficient at the region of excitation

• High quantum yield

• Optimal excitation wavelength

• Photostability

• Excited-state lifetime

• Minimal perturbation by probe

• Dye molecules must be close to but below saturation levels for optimum emission

• Fluorescence emission is longer than the exciting wavelength

• The energy of the light increases with reduction of wavelength

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Properties of Fluorescent Molecules

This slide covers the properties of fluorescent molecules. It emphasizes what properties need to be taken into consideration when choosing a fluorescent molecule for flow cytometry.