Fluorescence

• Excitation Spectrum

  • Intensity of emission as a function of exciting wavelength

• Chromophores are components of molecules which absorb light

• They are generally aromatic rings

• The wavelength of absorption is related to the size of the chromophores

• Smaller chromophores, higher energy (shorter wavelength)

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Fluorescence

This slide is of high importance in regards to staining. The aromatic rings relates the importance of taking into account the structure of the stain.